For assessment of Nuclear Biomarker Analysis
The algorithm identifies all nuclei in the blue plane of the image based on the intensity values for the hematoxylin counterstain and cells exhibiting nuclear positivity in the red plane of the image. Distance transform algorithm further applied on the segmented image yields more seed points to separate and segment as many connected cells as possible. An accurate view of nuclear scores mimicking the pathologist’s interpretation are generated.
This feature filters out residual or nonspecific staining in the cytoplasm or background automatically, separates positively stained nuclei from hematoxylin counterstain to generate reproducible and accurate results of true nuclear staining intensity.
The user can further train and tune the analysis parameters to determine inclusion of nuclei in analysis based on cell size, area, and staining intensity and evaluate multiple markers in a quantitative and high-throughput manner.